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KMID : 0381120070290030403
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Genes and Genomics
2007 Volume.29 No. 3 p.403 ~ p.407
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Transient Transfection Analysis of long Terminal Repeats of Porcine Endogenous Retroviruses in Sus Scrofa Coreanus
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Huh Jae-Won
Ha Hong-Seok
Kim Dae-Soo
Cho Byung-Wook
Kim Heui-Soo
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Abstract
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The characteristic phenotype of partial trisomy 14 includes growth and developmental Porcine endogenous retroviruses (PERVs) in the pig genome represent potential infectious risk factors in pig-to-human transplantation. Solitary long terminal repeat (LTR) elements of the PERV specifically affect the replication properties of single viruses and adjacent genes through their transcription factors. The promoter activity of solitary LTR elements belonging to the PERV-A and -B families in Korean wild pig (KWP) using a luciferase assay were examined. Five types of LTR structure (PERV-A5-KWP, PERV-A6-KWP1, PERV-A6-KWP2, PERV-A7-KWP, PERV-A8-KWP) showed different promoter activity in human HCT116 and COS7 cells. PERV-A6-KWP locus harbors the different copy number (seven or eight) of 39 bp repeats located in U3 region and showed different promoter activity. Our data suggests that copy numbers of 39 bp repeats and specific insertion and deletion (INDEL) sequences in the U3 region of a solitary LTR element could regulate the different transcription activity in pig genome.
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KEYWORD
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PERV, LTR element, promoter, Sus scrofa coreanus
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